International Journal of Immunology is a peer-reviewed open access journal that publishes original research articles as well as review articles in all areas of immunology. Immunology is a diverse and growing discipline that can be defined as the study of the tissues, cells and molecules involved in host defense mechanisms. Immunologists attempt to understand how the immune system develops, how the body defends itself against disease, and what happens when it all goes wrong. Priority is given to work that provides fundamental insight into the workings of the immune system. Areas covered include, but are not limited to, innate immunity and inflammation; development; immune receptors, signaling and apoptosis; antigen presentation; gene regulation and recombination; cellular and systemic immunity; vaccines; immune tolerance; autoimmunity and tumor immunology, microbial immunopathology; and transplantation.

The great advances in immunology in recent years make this field one of the most dynamic and rapidly growing in biological sciences. This remarkable growth is stimulated by the influx of investigators from other disciplines such as biochemistry, genetics, molecular biology, microbiology, virology and various medical disciplines. These disciplines are so well interlinked with immunology that no immunological challenge can now be properly addressed without sophisticated applications of expertise of combinations of these disciplines. As a consequence, immunology has become a vast and rich field encompassing discoveries and outlooks that range from the highly clinical to the highly molecular. Although such perspectives may appear diverse, they are, in fact, extremely interdependent. The journal seeks to present a balanced overview of contemporary immunology and melds together different aspects of molecular immunology, immunobiology and clinical immunology.

Immunology is the branch of biomedical science that deals with the response of an organism to antigenic challenge and its recognition of what is self and what is not. It deals with the defense mechanisms including all physical, chemical and biological properties of the organism that help it to combat its susceptibility to foreign organisms, material, etc

Immunology has its origins in the study of how the body protects itself against infectious diseases caused by microorganisms, such as bacteria, viruses, protozoa, and fungi, and also parasitic organisms, such as helminth worms. Research / review articles deals with, among other things, the physiological functioning of the immune system in states of both health and disease; malfunctions of the immune system in immunological disorders (autoimmune diseases, hypersensitivities, immune deficiency, transplant rejection); the physical, chemical and physiological characteristics of the components of the immune system in vitro, in situ, and in vivo are of prime interest.




Girja Shukla

Associate Professor

Dr. Girja Shukla is an Associate Professor in the Department of Surgery and a Full Member of Cancer Center at the University of Vermont College of Medicine, Burlington, Vermont, USA. His major interest is to develop targeted therapy against breast cancer. It involves in vitro (fresh tumors, cancer cell lines, purified cancer targets) and in vivo (animals and cancer patients) selection of tumor targeting agents using phage-displayed peptide, single chain antibody and β-lactamase loop libraries. In addition, he is also interested in generating cancer-specific full-length human antibodies, using EBV immortalization and hybridoma techniques, from B cells obtained from sentinel nodes, bone marrow aspirates and tumor tissues of cancer patients. Recently, his group has been working on the high throughput immuno-sequencing of B-cell repertoire of tumor- and vaccine-draining lymph nodes from cancer patients and relevant preclinical models. He is also actively collaborating in several immunotherapy clinical trials being conducted at the University of Vermont, Dartmouth Hitchcock Medical Center, and University of Virginia.

BS (Major: Chemistry & Biology) 1972 Lucknow University
MS (Biochemistry) 1974 Lucknow University
Certificate in French Language 1977 Lucknow University
PhD (Biochemistry) 1981 Lucknow University

Cancer therapeutic and diagnostic antibodies, Tumor autoantibodies, High throughput sequencing of immune repertoire, cancer vaccine, Phage display for anticancer antibodies

Winner of prestigious National Young Scientist Award of Council of Scientific and Industrial Research (CSIR), Government of India, in 1989.

2005-Present            University of Vermont Cancer Center
2007-Present            American Association for Cancer Research
Life Member             Society of Biological Chemists (India)
Life Member             Indian Pharmacological Society
Life Member             Society of Toxicology (India)
Life Member             Indian Academy of Neuroscience
Life Member             Association of Physiologists and Pharmacologists of India

Editorial Board Member:
• Journal of Cancer Science & Therapy, one of the Open Access journals of OMICS Publishing Group, Foster City, CA
• European Journal of Toxicological Sciences, SCIK Publishing Corporation, London, UK.
• ISRN Toxicology, one of the International Scholarly Research Network (ISRN) series of journals, New York, NY
• World Journal of Biochemistry, a journal of Beshideng Publishing Group, Pleasanton, CA
• Mutations & Cancer Studies, a Scigmoid Group Publication, Shoshoni, WY
• Applied Scientific Reports, a Herbert publication, UK
• Journal of Spleen And Liver Research, a journal of Open Access Pub, Valley Cottage, NY
• Molecular Biology and Imaging, an Austin Publishing Group journal, Secaucus, NJ
• Case Reports: Open Access, a journal by J Scholar publishers, Dallas, TX
• SM Surgery Journal, an International Open Access journal, SM Online Publishers, Irving, TX
• Heliyon, an open access journal published by Elsevier, Amsterdam, The Netherlands.
• International Journal of Oncology Research, Clin Med Int Lib, Newark, DE, USA
• Current Trends in Vaccines and Vaccinology, Beverly Hills, California, USA
• Journal of Vaccines, Immunology and Immunopathology, Gavin Publishers, Lisle, IL, USA
• Virology & Immunology Journal, Medwin Publishers, Troy, MI, USA.
• Journal of Oncology and Cancer Research, Published by ZygoScient Research Insights, Inc., Los Angeles, CA, USA.

Publications: Total 105 publications; 100 Published Original Research Papers (+1 in-preparation); 2 Published Review papers; 3 Book/Monographs. 50 publications with first authorship, 4 with single authorship, 31 with corresponding/Senior authorship.

Original Research Publications:

1. Girja S. Shukla, S.V. Chandra and P.K. Seth (1976) Effect of manganese on the levels of DNA, RNA, DNAse and RNAse in cerebrum, cerebellum and rest of brain regions of rats.

 Acta Pharmacolica et Toxicologica 39: 562-569. PMID: 990038.

2. S.V. Chandra and Girja S. Shukla (1976) Role of iron deficiency in inducing susceptibility to manganese toxicity. Archives of Toxicology 35: 319-323. PMID: 989299.

3. Girja S. Shukla and S.V. Chandra (1976) Manganese-induced morphological and biochemical changes in the brain of iron-deficient rats. Industrial Health 14: 87-92.

4. Girja S. Shukla and S.V. Chandra (1977) Levels of sulfhydryls and sulfhydryl-containing enzymes in brain, liver and testis of manganese treated rats. Archives of Toxicology 17: 319-325. PMID: 578708.

5. S.V. Chandra and Girja S. Shukla (1977) Effect of manganese administration on morphology and enzymes of brain in iron-deficient rats. Environmental Pollution & Health 16: 172-177.

6. S.V. Chandra and Girja S. Shukla (1978) Manganese encephalopathy in growing rats.

Environmental Research 15: 28-37. PMID: 620661.

7. Girja S. Shukla, S. Singh and S.V. Chandra (1978) Interaction between manganese and ethanol in rats. Acta Pharmacologica et Toxicologica 43: 354-362. PMID: 153083.

8. Girja S. Shukla and S.V. Chandra (1979) Species variation in manganese-induced changes in brain biogenic amines. Toxicology Letters 3: 249-253.

9. S. Singh, Girja S. Shukla, R. S. Srivastava and S.V. Chandra (1979) The interaction between ethanol and manganese in rat brain. Acta Pharmacologica et Toxicologica 41: 307-316. PMID: 435081.

10. S.V. Chandra and Girja S. Shukla (1979) Regional distribution of metals and biogenic amines in the brain of monkeys exposed to manganese. Toxicology Letters 4: 189-192.

11. S.V. Chandra, Girja S. Shukla and R.C. Murthy (1978) Effect of stress on the response of rat brain to manganese. Toxicology & Applied Pharmacology 47: 499-504. PMID: 442097.

12. Girja S. Shukla and S.V. Chandra (1978) Effect of nickel on aromatic amino acids and biogenic amines in brain of guinea pigs. Toxicology Letters 3: 270-272.

13. S.V. Chandra, Girja S. Shukla and D.K. Saxena (1979) Manganese-induced behavioral dysfunction and its neurochemical mechanism in growing mice. Journal of Neurochemistry 33: 1217-1221. PMID: 552401.

14. S.V. Chandra, Girja S. Shukla, R.S. Srivastava, S.K. Gupta (1980) Combined effects of metals on biogenic amines and their distribution in brain of mice. Archives of Environmental Contamination & Toxicology 9: 79-87. PMID: 7369787.

15. Girja S. Shukla, M.P. Dubey and S.V. Chandra (1980) Manganese-induced biochemical changes in growing versus adult rats. Archives of Environmental Contamination & Toxicology 9: 79-85. PMID: 6105852.

16. S.V. Chandra, Girja S. Shukla, R.S. Srivastava, H. Singh and V.P. Gupta (1981) An exploratory study of manganese exposure to welders. Clinical Toxicology 9: 407-418. PMID: 7237969.

17. Girja S. Shukla and S.V. Chandra (1981) Manganese toxicity: Lipid peroxidation in rat brain.

      Acta Pharmacolica et Toxicologica 48: 95-100. PMID: 7257834.

18. S.V. Chandra and Girja S. Shukla (1981) Concentration of striatal catecholamines in rats given manganese chloride through drinking water. Journal of Neurochemistry 36: 683-687. PMID: 7463083.

19. Girja S. Shukla and S.V. Chandra (1981) Striatal dopamone turnover and L-dopa treatment after short-term exposure of rats to manganese. Archives of Toxicology 47: 191-196. PMID: 7271447.

20. M.M. Ali, D.K. Saxena, Girja S. Shukla and S.V. Chandra (1981) Behavioral dysfunctions and central neurotransmitters in manganese exposed rats. Journal of Environmental Biology 2: 29-39.

21. R.C. Murthy, S. Lal, D.K. Saxena, Girja S. Shukla, M.M. Ali, and S.V. Chandra (1981) Effect of manganese and copper interaction on behavior and biogenic amines in rats fed a 10% casein diet. Chemical-Biological Interaction 37: 299-308. PMID: 7296699.

22. Girja S. Shukla and S.V. Chandra (1982) Effect of interaction of manganese with zinc, mercury and cadmium on some neurochemicals in rats. Toxicology Letters 10: 163-168. PMID: 6177064.

23. S.V. Chandra and Girja S. Shukla (1982) Effect of manganese on synthesis of brain catecholamines in growing rats. Acta Pharmacologica et Toxicologica 51: 209-216. PMID: 7336950.

24. K.M. Malhotra, Girja S. Shukla and S.V. Chandra (1982) Neurochemical changes in the rats coexposed to lead and copper. Archives of Toxicology 49: 331-336. PMID: 6284090.

25. Girja S. Shukla and S.V. Chandra (1982) Effect of manganese on carbohydrate metabolism and mitochondrial enzymes in rats. Archives of Toxicology 51: 209-216. PMID: 7136726.

26. S.V. Chandra, K.M. Malhotra and Girja S. Shukla (1982) GABArgic neurochemistry in manganese exposed rats. Acta Pharmacologica et Toxicologica 51: 456-458. PMID: 7164827.

27. Girja S. Shukla and S.V. Chandra (1983) Early postnatal exposure to manganese in rats: Effect on brain weight, 54manganese uptake and biogenic amines. Journal of Environmental Biology 4: 175-184.

28. Girja S. Shukla, K.M. Malhotra and S.V. Chandra (1983) Effect of manganese on rat brain microsomal (Mg2+, Na+, K+) ATPases: In vitro and in vivo studies. Environmental Research 32: 212-219. PMID: 6137360.

29. K. Kalia, Girja S. Shukla and S.V. Chandra (1984) Lead and copper interaction: Hematocrit, ALAD and ceruloplasmin in rats. Advances in Bioscience 3: 75-79. 

30. Girja S. Shukla and R.L. Singhal (1985) Hormone-independent polyamine metabolism of squamous cell carcinoma of the prostate. Cell Biochemistry & Function 3: 185-192. PMID: 3836020.

31. Girja S. Shukla (1985) Combined lithium and valproate treatment and subsequent withdrawal: seratonergic mechanism of their interaction in discrete brain regions. Progress in Neuro-psychopharmacology & Biological Psychiatry 9: 153-156. PMID: 3923559.

32. Girja S. Shukla (1987) Mechanism of lithium interaction: In vivo and in vitro effects of alkali metals on brain superoxide dismutase. Pharmacology Biochemistry & Behavior 26: 235-240. PMID: 3033694.

33. Girja S. Shukla (1987) GABAergic mechanism of interaction of lithium and valproate in discrete rat brain regions following their combined treatment and subsequent withdrawal. Drug Development Research 11: 209-218.

34. Girja S. Shukla and S.V. Chandra (1987) Concurrent exposure of lead, manganese and cadmium and their distribution in various brain regions, liver, kidney and testis of growing rats. Archives of Environmental Contamination & Toxicology 16: 303-310. PMID: 3592755.

35. T. Hussain, Girja S. Shukla and S.V. Chandra (1987) Effect of cadmium on superoxide dismutase and lipid peroxidation in liver and kidney of rats: In vivo and in vitro studies.

Pharmacology & Toxicology 60: 355-359. PMID: 3615346.

36. Girja S. Shukla, R.S. Srivastava and S.V. Chandra (1987) Glutathione metabolism in liver, kidney and testis of rats exposed to cadmium. Industrial Health 21: 139-145. PMID: 3679890.

37. Girja S. Shukla, T. Hussain and S.V. Chandra (1987) Possible role of superoxide dismutase activity and lipid peroxide levels in cadmium neurotoxicity: In vivo and in vitro studies in growing rats. Life Science 14: 2215-2223. PMID: 3669920.

38. Girja S. Shukla, K. Kalia and S.V. Chandra (1988) Age dependent distribution of 109cadmium in selected organs of rat. Chemosphere 17: 661-670. PMID: 3220202.

39. Girja S. Shukla, R.S. Srivastava and S.V. Chandra (1988) Glutathione status and cadmium neurotoxicity: Studies in discrete brain regions of growing rats. Fundamental & Applied Toxicology 11: 229-235. PMID: 3220202.

40. Girja S. Shukla, T. Hussain and S.V. Chandra (1988) Protective effect of vitamin E on cadmium-induced alterations in lipofuscin and superoxide dismutase in rat brain regions.

Pharmacology & Toxicology 63: 305-306. PMID: 3194351.

41. Girja S. Shukla, R.S. Srivastava and S.V. Chandra (1988) Prevention of cadmium-induced effects on regional glutathione status of brain by vitamin E. Journal of Applied Toxicology 8: 355-359. PMID: 3230246.

42. Girja S. Shukla, T. Hussain, R.S. Srivastava and S.V. Chandra (1988) Diagnostic significance of erythrocyte antioxidative enzymes in cadmium toxicity. Biochemical Archives 4: 429-436.

43. Girja S. Shukla, T. Hussain and S.V. Chandra (1988) Protein malnourishment alters organ distribution of 54manganese in rats. Biochemical Archives 4: 151-157.

44. Girja S. Shukla, T. Hussain, R.S. Srivastava and S.V. Chandra (1989) Glutathione peroxidase and catalase in liver, kidney, testis and brain regions of rats following cadmium exposure and subsequent withdrawal. Industrial Health. 27: 59-69. PMID: 2745162.

45. Girja S. Shukla and S.V. Chandra (1989) Cadmium toxicity and bioantioxidants: Status of vitamine E and ascorbic acid of selected organs. Journal of Applied Toxicology 9: 119-122. PMID: 2715567.

46. A. Shukla, K.N. Agarwal and Girja S. Shukla (1989) Latent iron deficiency alters - aminobutyric acid and glutamate metabolism in rat brain. Experientia 45: 343-345. PMID: 2565248.

47. A. Shukla, K.N. Agarwal and Girja S. Shukla (1989) Effect of latent iron deficiency on metal levels of rat brain regions. Biological Trace Element Research 22: 141-152. PMID: 2484235.

48. A. Shukla, K.N. Agarwal and Girja S. Shukla (1989) Rat brain catecholamines in latent iron deficiency. Nutrition Research 9: 1177-1186.

49. N.S. Radin, A. Shukla, Girja S. Shukla and A. Sano (1989) Heat-stable protein that stimulate -glucosidase. Biochemical Journal 264: 845-849. PMID: 2695068; PMCID: PMC1133662.

50. A. Shukla, K.N. Agarwal and Girja S. Shukla (1990) Effect of latent iron deficiency on the levels of iron, calcium, zinc, copper, manganese, cadmium and lead in liver, kidney and spleen of growing rats. Experientia 46: 751-752. PMID: 2565248.

51. Girja S. Shukla and N.S. Radin (1990) Glucosylceramide synthase of mouse kidney: Further characterization and improved assay. Archives of Biochemistry & Biophysics 283: 272-278. PMID: 2148864.

52. N.S. Radin and Girja S. Shukla (1991) Ultrasonic baths as substitutes for shaking incubator baths. Enzyme 45: 67-70. PMID: 1806368.

53. Girja S. Shukla and A. Shukla and N.S. Radin (1991) Gangliosides inhibit glucosylceramide synthase: Possible role in ganglioside therapy. Journal of Neurochemistry 56: 2125-2132. PMID: 1827496.

54. Girja S. Shukla and A. Shukla and N.S. Radin (1991) Rapid kidney changes resulting from glycosphingolipid depletion by treatment with a glucosyltransferase inhibitor. Biochem Biophys Acta 1083: 101-108. PMID: 1827738.

55. A. Shukla, Girja S. Shukla and N.S. Radin (1992) Control of kidney size by sex hormones: possible role of involvement of glucosylceramide. American Journal of Physiology 262: F24-29. PMID: 1733293.

56. A. Abe, J. Inokuchi, M. Jimbo, H. Shimeno, A. Nagamatsu, J.A. Shayman, Girja S. Shukla and N.S. Radin (1992) Improved inhibitors of glucosylceramide synthase. Journal of Biochemistry 111: 191-196. PMID: 1533217.

57. M.M. Ali, Girja S. Shukla, R.S. Srivastava, N. Mathur and S.V. Chandra (1993) Vitamin E prevents cadmium-induced locomotor behavior dysfunction in rat. Veterinary & Human Toxicology 35: 109-111. PMID: 8470351.

58. A. Gupta, A. Gupta and Girja S. Shukla (1995) Development of brain free radical scavenging system and lipid peroxidation under the influence of gestational and lactational cadmium exposure. Human & Experimental Toxicology 14: 428-433. PMID: 7612305.

59. A. Gupta and Girja S. Shukla (1995) Effect of chronic aluminum exposure on the levels of conjugated dienes and enzymatic antioxidants in hippocampus and whole brain of rat. Bulletin of Environmental Contamination & Toxicology 55: 716-722. PMID: 8563205.

60. A. Shukla, Girja S. Shukla and R.C. Srimal (1996) Cadmium-induced alteration in blood-brain barrier permeability and its possible correlation with decreased microvessel antioxidant potential in rat. Human & Experimental Toxicology 15: 400-405. PMID: 8735464.

61. A. Gupta, A. Gupta and Girja S. Shukla (1996) Maternal cadmium intoxication: effects of fetal brain antioxidants defense parameters. Redox Report 2: 199-204. PMID: 27406077.

62. A. Gupta and Girja S. Shukla (1996) Ontogenic profile of brain lipids following perinatal exposure to cadmium. Journal of Applied Toxicology 16: 227-233. PMID: 8818863.

63. A. Gupta and Girja S. Shukla (1997) Enzymatic antioxidants in erythrocytes following heavy metal exposure: Possible role in early diagnosis of the exposure. Bulletin of Environmental Contamination & Toxicology 58: 198-205. PMID: 8975794.

64. A. Gupta, A. Gupta and Girja S. Shukla (1998) Effects of neonatal quinalphos exposure and subsequent withdrawal on free radical generation and antioxidative defenses in developing rat brain. Journal of Applied Toxicology 18: 71-77. PMID: 9467831.

65. R.R. Gupta, C.G. Agrawal, Girja S. Shukla, B. Ali (1998) Cytochrome P450-dependent oxidation and glutathione conjugation of xenobiotics in alloxan-induced diabetic rat. Research Communications in Molecular Pathology & Pharmacology 98: 231-236. PMID: 9467831.

66. D. Nigam, A. Gupta, Girja S. Shukla, and A.K. Agarwal (1999) Glutathione depletion and oxidative damage in mitochondria following exposure to cadmium in rat liver and kidney. Toxicology Letters 106: 151-157. PMID: 10403659. 

67. R. Agarwal and Girja S. Shukla (1999) Potential role of cerebral glutathione in the maintenance of blood-brain barrier integrity in rat. Neurochemical Research 24: 1507-1514. 10568684.

68. R. Agarwal, A. Gupta and Girja S. Shukla (1999) Developmental pattern of reactive oxygen species generation and antioxidant defense machinery in rat cerebral microvessels.

International Journal of Developmental Neuroscience 17: 673-679. PMID: 10568684.

69. A. Gupta, A. Gupta, D. Nigam, Girja S. Shukla and A.K. Agarwal (1999) Profile of reactive oxygen species generation and antioxidative mechanisms in the maturing rat kidney. Journal of Applied Toxicology 19: 55-59. PMID: 9989478.

70. A. Gupta, R. Agarwal and Girja S. Shukla (1999) Functional impairment of blood-brain barrier following pesticide exposure during early development in rats. Human & Experimental Toxicology 18: 174-179. PMID: 10215108.

71. A. Gupta, D. Nigam, A. Gupta, Girja S. Shukla and A.K. Agarwal (1999) Effect of pyrethroid based liquid mosquito repellent inhalation on the bood-brain barrier function and oxidative stress in selected organs of developing rats. Journal of Applied Toxicology 19: 67-72. PMID: 9989480.

72. B.A. Hart, C.H. Lee, Girja S. Shukla, A. Shukla, M. Osier, J.D. Eneman and J-F Chiu (1999) Characterization of cadmium-induced apoptosis in rat lung epithelial cells: Evidence for the participation of oxidant stress. Toxicology 133: 43-58. PMID: 10413193.

73. A. Gupta, D. Nigam, A. Gupta, A.K. Agarwal and Girja S. Shukla (2000) Effect of quinalphos and cypermethrin exposure on developing blood-brain barrier: Possible role of nitric oxide. Environmental Toxicology & Pharmacology 8: 73-78. PMID: 10867365.

74. R. Agarwal, A. Gupta, M. Datta and Girja S. Shukla (2000) Cerebral antioxidant status and free radical generation following glutathione depletion and subsequent recovery. Molecular & Cellular Biochemistry 209: 55-61. PMID: 10942201.

75. Girja S. Shukla, A. Shukla, R.J. potts, M. Ozier, B.A. hart and J-F Chiu (2000) Cadmium-mediated oxidative stress in alveolar epithelial cells induces the expression of γ-glutamylcysteine synthetase catalytic subunit and glutathione S-transferase α and π isoforms: Potential role of activator Protein-1. Cell Biology & Toxicology 16: 347-362. PMID: 11254161.

76. Girja S. Shukla, J-F Chiu and B.A. Hart (2000) Cadmium-induced elevations in the gene expression of the regulatory subunit of γ-glutamylcysteine synthetase in rat lung and alveolar epithelial cells. Toxicology 151: 45-54. PMID: 11074299.

77. J.D. Eneman, R.J. Potts, M. Osier, Girja S. Shukla, J-F Chiu and B.A. Hart (2000) Suppressed oxidant induced apoptosis in cadmium adapted alveolar epithelial cells and its potential involvement in cadmium carcinogenesis. Toxicology 147: 215-228. PMID: 10924803.

78. Girja S. Shukla, J-F Chiu and B.A. Hart (2000) Enhanced expression of pulmonary - glutamylcysteine synthetase heavy subunit in rats exposed to cadmium aerosols. Toxicology & Applied Pharmacology 163: 249-259. PMID: 10702364.

79. Chaitali Sinha and Girja S. Shukla (2003) Species variation in pesticide-induced blood-brain barrier dysfunction. Human & Experimental Toxicology 22: 647-652. PMID: 14992326.

80. Stephanie C. Pero, Girja S. Shukla, Amy L. Armstrong, Daniel Peterson, Susan P. Fuller, Katherine Godin, Sarah L. Kingsley, Richards, Donald L. Weaver, Jeffrey Bond and David N. Krag (2004) Identification of a small peptide that inhibits the phosphorylation of ErbB2 and proliferation of ErbB2 overexpressing breast cancer cells. International Journal of Cancer 111(6): 951-60. PMID: 15300809.

81. Girja S. Shukla and David N. Krag (2005) A sensitive and rapid chemiluminescence ELISA for filamentous bacteriophages. Journal of Immunoassay & Immunochemistry 26: 89-95. PMID: 15794119.

82. Girja S. Shukla and David N. Krag (2005) Phage display selection for cell-specific ligands: development of screening procedure suitable for small tumor specimen. Journal of Drug Targeting 13 (1): 7-18. PMID: 15848950.

83. Girja S. Shukla and David N. Krag (2005) Selection of tumor-targeting agents on freshly excised human breast tumors using a phage display library. Oncology Reports 13 (4): 757-764. PMID: 17756454.

84. David N. Krag, Girja S. Shukla, Guang-Ping Shen, Stephanie Pero, Susan Fuller, Donald L. Weaver, Susan Burdette-Radoux and Christian Thomas (2006) Selection of tumor-binding ligands in cancer patients with phage display libraries. Cancer Research 66 (15): 7724-7733. PMID: 16885375.

85. Girja S. Shukla, Christopher J. Murray, Melodie Estabrook, Guang-Ping Shen, Volker Schellenberger and David N. Krag (2007) Towards a ligand targeted enzyme prodrug therapy: Single round panning of a β-lactamase scaffold library on human cancer cells. International Journal of Cancer 120: 2233-2242. PMID: 17285581.

86. Stephanie Pero, Girja S. Shukla, Michelle Cookson, Stevenson Flemer Jr., and David N. Krag (2007) Combination treatment with Grb7 peptide and Doxorubicin or Trastuzumab (Herceptin) results in cooperative cell growth inhibition in breast cancer cells. British Journal of Cancer 96: 1520-1525. PMID: 17426702; PMCID: PMC2359946.

87. Yujing Sun, Girja S. Shukla, Guy G. Kennedy, David M. Warshaw, Donald L. Weaver, Stephanie Pero, Lisa Floyd, and David N. Krag (2009) Biopanning phage-display libraries on small tissue sections captured by laser capture microdissection. Journal of Biotech Research, 1(2): 55-63. PMID: 21822461; PMCID: PMC3149830.

88. Yujing Sun, Girja S. Shukla, Donald Weaver, Stephanie Pero, David N. Krag (2010) Phage-display selection on tumor histological specimens with laser capture microdissection. Journal of Immunological Methods, 347: 46-53. PMID: 19538966; PMCID: PMC2824587.

89. Girja S. Shukla and David N. Krag (2009) Developing bifunctional β-lactamase molecules with built-in target-recognizing module for prodrug therapy: Identification of Enterobacter cloacae P99 cephalosporinase loops suitable for randomization and phage-display selection. Journal of Molecular Recognition, 9 (6): 425-436. PMID: 19437416; PMCID: PMC2824592.

90. Girja S. Shukla and David N. Krag (2010) Phage-displayed combinatorial peptide libraries in fusion to β-lactamase as reporter for an accelerated clone screening: Potential uses of selected enzyme-linked affinity reagents in downstream applications. Combinatorial Chemistry and High Throughput Screening, 13 (1): 75-87. PMID: 20214576; PMCID: PMC2864580.

91. Girja S. Shukla and David N. Krag (2010) Novel β-lactamase-random peptide fusion libraries for phage display selection of cancer cell-targeting agents suitable for enzyme prodrug therapy. Journal of Drug Targeting, 18 (2): 115-124. PMID: 19751096; PMCID: PMC2877152.

92. Girja S. Shukla and David N. Krag (2010) Cancer cell -specific internalizing ligands from phage displayed β-lactamase-peptide fusion libraries. Protein Engineering, Design & Selection, 23 (6): 431-40. PMID: 20219829; PMCID: PMC2865360.

93. Nigus Ambaye, S.C. Pero, Menachem Gunzburg, MinYin Yap, Daniel Clayton, Mark Del Borgo, Patrick Perlmutter, Marie-Isabel Aguilar, Girja S. Shukla, Elena Peletskaya, Michelle Cookson, David N. Krag, Matthew Wilce and Jacqueline Wilce (2011) Structural basis of binding by cyclic nonphosphorylated peptide antagonists of grb7 implicated in breast cancer progression. Journal of Molecular Biology, 412 (3): 397-411. PMID: 21802427.

94. Yujing Sun, Girja S. Shukla, S.C. Pero, E. Currier, G. Sholler, D.N. Krag. (2012)  Single  tumor imaging with multiple antibodies targeting different antigens. Biotechniques. Apr 1: 52(4). doi: 10.2144/000113855. PMID: 26307247.

95. Girja S. Shukla, David N. Krag, Elena N. Peletskaya, Yujing Sun, Stephanie C. Pero, Laurence E. McCahill, and Thomas A. Roland (2013) Intravenous infusion of phage-displayed antibody library in human cancer patients: Enrichment and cancer-specificity of tumor-homing phage-antibodies. Cancer Immunology, Immunotherapy, 62:1397-1410. PMID: 23736951: PMCID: PMC4524473.

96. Yujing Sun, G.S. Sholler, Girja S. Shukla, Stephanie C. Pero, Chelsea L. Carman, Ping Zhao, David N. Krag (2016) Autologous antibodies that bind neuroblastoma cells. Journal of Immunological Methods, 426:35-41. PMID: 26210205.

97. S.C. Pero, Yujing Sun, Girja S. Shukla, Chelsea L. Carman, Christopher C. Krag, Cory Teuscher, Dimitry N. Krementsov, David N. Krag (2017) Vaccine draining lymph nodes are a source of antigen-specific B cells. Vaccine, 35:1259-1265. PMID: 28161423.

98. Girja S. Shukla, Walter C. Olson, Stephanie C. Pero, Yujing Sun, Chelsea L. Carman, Craig L. Slingluff, Jr., David N. Krag (2017) Vaccine-draining lymph nodes of cancer patients for generating anti-cancer antibodies. Journal of Translational Medicine, 15:180 (DOI10.1186/s12967-017-1283-8). PMID: 28851380; PMCID: PMC5575880.

99. J.R. McDaniel, S.C. Pero, W.N. Voss, Girja S. Shukla, Yujing Sun, Sebastian Schaetzle, Chang-Han Lee, Andrew P. Horton, Seth Harlow, Jimmy Gollihar, Jared W. Ellefson, Christopher C. Krag, Yuri Tanno, Nikoletta Sidiropoulos, George Georgiou, Gregory C. Ippolito, David N. Krag (2017) High-throughput discovery of antitumor antibodies mined from sentinel lymph node B cells and circulating IgG of breast cancer patients. Cancer Immunology Immunotherapy (Accepted).

100. Girja S. Shukla, Stephanie C. Pero, Yu-Jing Sun, Chelsea Carman, Seth Harlow and David N. Krag (2017) Characterization of Sentinel Node-Derived Antibodies from Breast Cancer Patients. Journal of Immunological Methods (Provisionally accepted).

Peer-reviewed review articles:

101. Girja S. Shukla and R.L. Singhal (1984) The Present status of biological effects of toxic metals in the environment: Lead, cadmium and manganese. Canadian Journal of Physiology & Pharmacology 62: 1015-1031. PMID: 6149004.

102. Girja S. Shukla and David N. Krag (2006) Selective delivery of therapeutic agents in diagnosis and treatment of cancer. Expert Opinion in Biological therapy 6 (1): 39-54.

Books and Chapters:

103. R.L. Singhal, R. Vijayvargiya and Girja S. Shukla (1985) Toxic effects of cadmium and lead on reproductive functions. In: Toxicology of Endocrine System (Target Organ Toxicity series), Edited by J.A. Thomas, K.H. Korach and J. McLachlan. Raven Press, New York, NY, pp 149-179.

104. Girja S. Shukla (2009) Phage Display. In: Encyclopedia of Cancer, 2nd edition (ISBN 978-3-540-47648-1), Edited by Manfred Schwab, Springer Press, Heidelberg, Germany.

105. Girja S. Shukla (2017) Phage Display. In: Encyclopedia of Cancer, 4th edition (ISBN Print 978-3-662-46874-6, eBook 978-3-662-47424-2), Edited by Manfred Schwab, Springer Press, Heidelberg, Germany.